한세광 교수 연구실 (16.02)

admin | 2016.07.11 15:59 | 조회 151
Abstract

Vaccines are commonly administered by injection using needles. Although
transdermal microneedles are less invasive promising alternatives, needlefree
topical vaccination without involving physical damage to the natural skin
barrier is still sought after as it can further reduce needle-induced anxiety and
is simple to administer. However, this long-standing goal has been elusive
since the intact skin is impermeable to most macromolecules. Here, we
show an effi cient, noninvasive transdermal vaccination by employing two
key innovations: the use of hyaluronan (HA) as vaccine carriers and nonablative
laser adjuvants. Conjugates of a model vaccine ovalbumin (OVA) and
HA—HA–OVA conjugates—induced more effective maturation of dendritic
cells in vitro , compared to OVA. Following topical administration in the skin,
HA–OVA conjugates penetrated into the epidermis and dermis in murine and
porcine skins, as revealed by intravital microscopy and fl uorescence assay.
Topical administration of HA-OVA conjugates signifi cantly elevated both
humoral and mucosal antibodies, with peak levels at four weeks. An OVA
challenge at week eight elicited strong immune-recall responses. With pretreatment
of the skin using non-ablative fractional laser beams as adjuvant,
strong immunization was achieved with much reduced doses of HA–OVA
(1 mg kg –1 OVA). Our results demonstrate the potential of the noninvasive
patch-type transdermal vaccination platform.


==================================================
Noninvasive Transdermal Vaccination Using Hyaluronan Nanocarriers and Laser Adjuvant

==================================================

twitter facebook me2day 요즘